A primary photosynthetic reaction of phytoplankton in ecomonitoring

Matorin D.N., Antal T.K., Levenko B.A., Kazimirko Yu.V

Moscow State University, Bioplogical Faculty, Department of Biophysics, 119899, Vorobjovy Gory, Moscow, Russia

The efficiency of the primary reactions of photosynthesis in phytoplankton were studied under different environmental conditions by chlorophyll prompt and delayed fluorescence, and termoluminescence methods. The results of measuring of prompt and delayed fluorescence are used as an indicator of phytoplankton physiological state and for biomonitoring of environmental pollution. We have developed a set of computer-controlled devices for the chlorophyll luminescence analysis. It is the submerged pump and probe fluorometer to measure underwater illuminance, temperature and phytoplankton fluorescence parameters (Fo,Fv/Fm ) in situ. Maps of phytoplankton concentration and its photosynthetic activity distribution with depth in the Black Sea, Tirrenian Sea, Baikal lake, Norway sea are presented.

  1. Matorin D.N., Zakharkov S.P.,Venediktov P.S. (1988) Investigation of the high-temperature thermoluminescence of chlorophyll in marine phytoplankton. Sov. Plant Physiol. 35, N6, 1078-1084
  2. Matorin D.N.,Venedictov P.S.,(1990), Chlorophill luminescence in microalgae culture and natural phytoplankton populations, Itogy Nauki i Tekh.Ser.Biofiz., Moscow,40,40-101
  3. Matorin D.N., Vasil'ev I.R. and Vedernikov V.I. (1992) Photoinhibition of primary reactions of photosynthesis in natural populatins of phytoplankton in the Black Sea. Sov. Plant Physiol. 39(3),455-463.
  4. Matorin D.N., Venediktov P.S. Konev Yu.N., Kazimirko Yu.V., Rubin A.B. (1996) Application of a doublee-flash, impulse, submersible fluorimeter in the determination of photosynthetic activity of natural phytoplankton. Proc. Acad. Sci. USSR, Biol. Sect. 350(7): 1159-1161
  5. Matorin D.N.,Vavilin D.V, Konev Yu.N., Kazimirko Yu.V., Rubin A.B. (1997) A study on the correlation between pigment composition in microalgae and intencity of chlorophyll fluorescence measured with a pulse fluorometer. Moscow Univ. Biol. Bull. 16(1) 25-28
  6. Vavilin D.V., Vasil'ev I.R., Venediktov P.S, Izmest'eva L.R., Matorin D.N, and Polynov V.A.( 1993), Study of the state of Baikal phytoplankton by using chlorophyll luminescence, Hydrobiol. J. (Russia), 29(2), 3-12.
  7. Vavilin DV, Ducruet J-M, Matorin DN, Venedictov PS, Rubin AB (1997) Changes in Photosystem II activity, cell viability and the amplitude of high-temperature bands of chlorophyll thermoluminescence in green alga Chlorella pyrenoidosa subjected to various stress conditions. J. Photochem. Photobiol. B.Biology,42/3, 233-239