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Presentations

Ultrasound for monitoring and correction of hemostasis in vitro

Ivlev D.A.1, Dzhumaeva Sh.N.1, Uzlova S.G., Guria K.G.1

National research center for hematology, Russian Ministry of Health, laboratory of mathematical modeling of biological processes, Russia, 125167, Moscow, Noviy Zykovskiy pr., 4, tel.: +7 (915) 451-18-51, fax: +7 (495) 612-42-52, ivlev.d@blood.ru

1Moscow institute of physics and technology, Russia, 141701, Dolgoprudny, Institutsky per., 9

Hemostasis disorders are the main cause of heart attacks, strokes and pulmonary embolism. Tests, based on measurements of coagulation times, blood viscosity, dispersion and reflection of optical and acoustic signals, are used for hemostasis monitoring in modern clinical practice. In present work we studied capabilities of Doppler ultrasonic method for real-time monitoring of coagulation and fibrinolysis in vitro.

Fresh frozen plasma and whole blood from healthy donors were used in experiments. Blood circulation in closed system of silicon tubes was provided by peristaltic pump. The ultrasonic signal was detected by a scanner and then was processed and analyzed in real time by the specially designed software. The infusion of fibrinolytic agents into blood flow was performed by an automatic injector with adjustable parameters of injection. The injector operation was governed by a computer in accordance with the data of ultrasonic monitoring.

It was shown that suggested ultrasonic technique can be used for monitoring of coagulation and fibrinolysis in vitro. It was demonstrated that operative fibrinolytic injection (not later than 30 seconds after primary clots appearance) lead to dissolution of large clots. At the same time there was not significant fibrinolysis detected if the injection of fibrinolytic agents was performed with 5 minutes delay or more.

Taking into account that the developed method is principally non-invasive, it seems to be promising for the purposes of real-time clinical diagnostics.

Work was supported by the Russian Foundation for Basic Research (grants 14‑04‑01193 and 16‑34‑01180).

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